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Molecular Biology

Bio-Rad Real-Time PCR Video

Bio-Rad Real-Time PCR Video

Bio-Rad Real-Time PCR Video

Understanding the Real-Time PCR method.

DNA Tube Pyrosequencing Video

DNA Tube Pyrosequencing Video

DNA Tube Pyrosequencing Video

The technique was developed by Pål Nyrén and his student Mostafa Ronaghi at the Royal Institute of Technology in Stockholm in 1996.Procedure "Sequencing by synthesis" involves taking a single strand of the DNA to be sequenced and then synthesizing its complementary strand enzymatically.

ScienceLearn PFGE Video

ScienceLearn PFGE Video

ScienceLearn PFGE Video

ESR Scientist Dr Brent Gilpin explains how pulsed field gel electrophoresis is used to separate very large fragments of DNA. DNA from an organism is digested with an enzyme and run on a gel, which gives a barcode that is unique to that organism. This system is used by the PulseNet organisation to identify common sources of disease outbreaks.